Josef Mašek PhD
Head of the Department
Research activities
NANOPARTICLES AND NANOFIBRES AS CARRIERS OF ACTIVE SUBSTANCES
PREPARATION AND CHARACTERIZATION OF LIPOSOMES AND POLYMERAN NANOPARTICLES
LIPOSOM-BASED VACCINE FORMULATION AND TESTING
DRUG TARGETING, DIAGNOSTICS AND TERANOSTICS
DEVELOPMENT OF NEW ORAL MUCOADHESIVE DRUG FORMS
CONFOCAL MICROSCOPY
Project involvement
FIT (Pharmacology, Immunotherapy, nanoToxicology)
OP RDE project of the Ministry of Education, Youth and Sports of the Czech Republic – Reg.No. CZ.02.1.01/0.0/0.0/15_003/0000495
NV16-30299A - Nanoliposomal systems for rapid diagnosis of thrombi by MRI
AZV ČR NV16-30299A
Nanoliposomes for the development of recombinant vaccines and targeted immunotherapeutics
GAČR P304/10/1951 (2010-2013)
Multiepitope synthetic borreliosis vaccine for veterinary applications
TAČR TA01011165 (2011-2014)
Use of ultrasound in nanomedicine
KAN200520703 (2007-2011)
Bioactive biocompatible surfaces and new nanostructured composites for applications in medicine and pharmacy
KAN200100801 (2008-2012)
Center for studies of toxic properties of nanoparticles
CENATOX GBP503/12/G147 (2012 – 2018)
Education
2006–2013
Ph.D. in Biochemistry, Masaryk University Brno, Faculty of Science, Czech Republic
Dissertation thesis: ”Metallochelating nanoliposomes for construction of recombinant vaccines”
2009
Advanced Master´s degree (Doctor of Pharmacy – PharmD.) in Pharmacy, Pharmaceutical Technology and Galenic Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Pharmacy, Czech Republic
Advances Master´s thesis: “Optimization of ciclopiroxolamine dose and dissolution profile”
2005–2009
Bachelor´s degree (Bc.) in Cellular and Molecular Diagnostics, Masaryk University Brno, Faculty of Science, Czech Republic
Bachelor´s thesis: “RNA Interference in Gene Theraphy”
2001–2006
Master´s degree (Mgr.) in Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Pharmacy, Czech Republic
Awards
1st place in the “Discovery award” 2017
The award was given for an innovative achievement in the field of biomedicine for the work “Mucoadhesive nanofiber carrier of vaccines and therapeutic nanoparticles“
1st place in the poster section of the 5th Nano Today Conference 2017
„Sublingual delivery of therapeutic nanoparticles by means of multilayered nanofibre-based mucoadhesive film”, Hawaii, USA, 2017
1. place in the competition „Prix Sanofi de Pharmacie“, 2013
Special award in the 2013 Ministry of Agriculture Young Scientists Award
„Metallochelating nanoliposomes for construction of recombinant vaccines”, Praha, 2013
3rd place in the poster section of the international conference The 10th EFIS-EJI Tatra Immunology Conference 2012
„Preparation and characterisation of recombinant OSpC liposome-based experimental vaccine with entrapped lipophilic derivatives of norAbuMDP against Borrelia Burgdorferi”, Slovakia, 2012
1st place in the poster section of the international conference Nanocon 2009
“Preparation of monodisperse nickel-chelate nanoliposomes for immobilization of his-tag proteins: study of their structure using TEM, AFM and dynamic light scattering”, Rožnov pod Radhoštěm, 2009
Selected publications
Rational Design of Self-Emulsifying Pellet Formulation of Thymol: Technology Development Guided by Molecular-Level Structure Characterization and Ex Vivo Testing
J. Macku, K. Kubova, M. Urbanova, J. Muselik, A. Franc, G. Koutna, M. Pavelkova, D. Vetchy, J. Masek, E. Maskova, J. Brus, 2022: Pharmaceutics.
Controlled synthesis of gemini surfactant-capped gold nanoparticles. Gemini structure-nanoparticle properties relationship study
, 2022: Pharmaceutics.
Cytokine expression by CD163+ monocytes in healthy and Actinobacillus pleuropneumoniae-infected pigs
R. Jarosova, P. Ondrackova, L. Leva, K. Nedbalcova, M. Vicenova, J. Masek, J. Volf, J. Gebauer, T. Do, R. Guranc, Z. Sladek, J. Domingueze, M. Faldyna, 2022: Research in Veterinary Science.
Exploration of Neusilin ® US2 as an Acceptable Filler in HPMC Matrix Systems-Comparison of Pharmacopoeial and Dynamic Biorelevant Dissolution Study
, 2022: Pharmaceutics.
Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration
M. Pavelková, J. Vysloužil, K. Kubová, S. Pavloková, D. Molinková, V. Celer, A. Pechová, J. Mašek, D. Vetchý, 2021: Pharmaceutics.
Short-course sublingual immunotherapy by mucoadhesive patch and tolerogenic particle enhanced allergen presentation
P. Sørensen , P. Turánek-Knotigová, J. Mašek, J. Kotouček, F. Hubatka, E. Mašková, P. Kulich, D. Lubasová, M. Raška, K. Leenhouts, J. Turánek , 2021: Clinical & Experimental Allergy.
Ni and TiO 2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts
M. Štefančík, L. Válková, J. Veverková, J. Balvan, T. Vičar, P. Babula, J. Mašek, P. Kulich, M. Pávková Goldbergová, 2021: Environmental Science and Pollution Research.
A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype
M. Hýžďalová, J. Procházková, S. Strapáčová, L. Svržková, O. Vacek, R. Fedr, Z. Andrysík, E. Hrubá, H. Líbalová, J. Kléma, J. Topinka, J. Mašek, K. Souček, J. Vondráček, M. Machala, 2021: Chemosphere.
Myomedin scaffold variants targeted to 10E8 HIV-1 broadly neutralizing antibody mimic gp41 epitope and elicit HIV-1 virus-neutralizing sera in mice
M. Kuchař, P. Kosztyu, V. Daniel Lišková, J. Černý, H. Petroková, E. Vróblová, M. Malý, L. Vaňková, M. Křupka, L. Rašková Kafková, P. Turánek Knotigová, J. Dušková, J. Dohnálek, J. Mašek, J. Turánek, M. Raška, P. Malý, 2021: Virulence.
Targeting Human Thrombus by Liposomes Modified with Anti-Fibrin Protein Binders
Petroková H., Mašek J., Kuchař M., Vítečková Wünschová A.,Štikarová J., Bartheldyová E., Kulich P., Hubatka F., Kotouček J., Knotigová P.T., Vohlídalová E., Héžová R., Mašková E., Macaulay S., Dyr J.E., Raška M., Mikulík R., Malý P., Turánek J., 2019. Pharmaceutics 11(12), 642; https://doi.org/10.3390/pharmaceutics11120642.
Proteins mimicking epitope of HIV-1 virus neutralizing antibody induce virus-neutralizing sera in mice
Kosztyu P., Kuchar M, Cerny J., Barkocziova L., Maly M., Petrokova H., Czernekova L., Liskova V., Raskova Kafkova L., Knotigova P., Masek J., Turanek J., Maly P., Raska M., 2019. EBioMedicine 47, 247-256. doi: 10.1016/j.ebiom.2019.07.015.
Application of Advanced Microscopic Methods to Study the Interaction of Carboxylated Fluorescent Nanodiamonds with Membrane Structures in THP-1 Cells: Activation of Inflammasome NLRP3 as the Result of Lysosome Destabilization
Turánek J., Knötigová P.T., Mašek J., Hubatka F., Kulich P., Kotouček J., Šimečková P., Čelechovská H., Koudelka Š., Procházka L., Bartheldyová E., Héžová R., Švadláková T., Vaškovicová N., Skoupý R., Krzyžánek V., Macaulay S., Fekete L., Machala M., Raška M., Kratochvílová I., 2019. Molecular Pharmaceutics, 2019, 168, 3441-3451.
N-oxy lipid-based click chemistry for orthogonal coupling of mannan onto nanoliposomes prepared by microfluidic mixing: synthesis of lipids, characterisation of mannan-coated nanoliposomes and in vitro stimulation of dendritic cells
Bartheldyová E., Knötigová P.T., Zachová K., Mašek J., Kulich P., Effenberg R., Zyka D., Hubatka F., Kotouček J., Čelechovská H., Héžová R., Tomečková A., Mašková E., Fojtikova M., Macaulay S., Bystrický P., Paulovičová L., Paulovičová E., Droz L., Ledvina M., Raška M., Turánek J., 2019. Carbohydrate Polymers 207, 521-532.
Hyaluronic Acid Surface Modified Liposomes Prepared via Orthogonal Aminoxy Coupling: Synthesis of Nontoxic Aminoxylipids Based on Symmetrically α-Branched Fatty Acids, Preparation of Liposomes by Microfluidic Mixing, and Targeting to Cancer Cells Expressing CD44
Bartheldyová E., Effenberg R., Mašek J., Procházka L., Knötigová P.T,, Kulich P., Hubatka F., Velínská K., Zelníčková J., Zouharová D., Fojtíková M., Hrebík D., Plevka P., Mikulík R., Miller A.D., Macaulay S., Zyka D., Drož L., Raška M., Ledvina M., Turánek J., 2018. Bioconjug Chem 29(7), 2343-2356.
doi:10.1021/acs.bioconjchem.8b00311.
Nonpyrogenic Molecular Adjuvants Based on norAbu-Muramyldipeptide and norAbu-Glucosaminyl Muramyldipeptide: Synthesis, Molecular Mechanisms of Action, and Biological Activities in Vitro and in Vivo
Effenberg R., Knotigova P.T., Zyka D., Celechovska H., Masek J., Bartheldyova E., Hubatka F., Koudelka S., Lukac R., Kovalova A., Saman D., Krupka M., Barkocziova L., Kosztyu P., Sebela M., Droz L., Hucko M., Kanasova M., Miller A.D., Raska M., Ledvina M., Turanek J. 2017. Journal of Medicinal Chemistry 60, 7745-7763.
Multi-layered nanofibrous mucoadhesive films for buccal and sublingual administration of drug-delivery and vaccination nanoparticles – important step towards effective mucosal vaccines
Mašek J., Lubasová D., Lukáč R., Turánek-Knotigová P., Kulich P., Plocková J.,
Mašková E., Procházka L., Koudelka Š., Sasithorn N., Gombos J., Bartheldyová E., Hubatka F., Raška M., Miller A.D., Turánek J., 2017. J Control Release 249, 183–195.
The Position of His-Tag in Recombinant OspC and Application of Various Adjuvants Affects the Intensity and Quality of Specific Antibody Response after Immunization of Experimental Mice
Krupka M., Masek J., Barkocziova L., Knotigova P.T., Kulich P., Plockova J., Lukac R., Bartheldyova E., Koudelka S., Chaloupkova R., Sebela M., Zyka D., Droz L., Effenberg R, Ledvina M, Miller AD, Turanek J, Raska M., 2016. Plos One 11, Article Number: e0148497.
Molecular Adjuvants Based on Nonpyrogenic Lipophilic Derivatives ofnorAbuMDP/GMDP Formulated in Nanoliposomes: Stimulation of Innate and Adaptive Immunity
Knotigova P.T., Zyka D., Masek J., Kovalova A., Krupka M., Bartheldyova E., Kulich P., Koudelka S., Lukac R., Kauerova Z., Vacek A., Horynova M.S., Kozubik A., Miller A.D., Fekete L., Kratochvilova I., Jezek J., Ledvina M., Raska M., Turanek J., 2015. Pharmaceutical Research 32, 1186-1199.
Enhancement of immune response towards non-lipidized Borrelia burgdorferi recombinant OspC antigen by binding onto the surface of metallochelating nanoliposomes with entrapped lipophilic derivatives of norAbuMDP
Krupka M., Masek J., Bartheldyova E., Knotigova P.T., Plockova J., Korvasova Z., Skrabalova M., Koudelka S., Kulich P., Zachova K., Czernekova L., Strouhal O., Horynova M., Sebela M., Miller A.D., Ledvina M., Raska M., Turanek J., 2012. J Control Release 160, 374-381.
Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged antigens: Preparation, structural study and immune response towards rHsp90
Masek J., Bartheldyova E., Turanek-Knotigova P., Skrabalova M., Korvasova Z., Plockova J., Koudelka S., Skodova P., Kulich P., Krupka M., Zachova K., Czernekova L., Horynova M., Kratochvilova I., Miller A.D., Zyka D., Michalek J., Vrbkova J., Sebela M., Ledvina M., Raska M., Turanek J., 2011. J Control Release 151, 193-201.
Book chapters
Nanofibers in Mucosal Drug and Vaccine Delivery
Mašek J., Mašková E., Lubasová D., Špánek R., Raška M, Turánek J. In: Morari Do Nascimento, G. (Ed.) Nanofibers – from Preparation to Applications. 2019, ISBN 978-953-51-6328-2.
Binding of Polysaccharides onto Liposomes and Characterization of Liposomal Preparation by Physical-Chemical and Advanced Microscopic Methods
Turanek J., Masek J., Raska M., Ledvina M., Paulovicova E., Hubatka F.,
Kotoucek J. In: Maiti, S.: Functional Polysaccharides for Biomedical Application. Elsevier, 2019.
Lipid-Based Nanoparticles and Microbublles
Turanek J., Miller A.D., Kauerová Z., Lukáč R., Masek J., Koudelka S., Raska M. In: Serra, PA: Advances in Bioengeneering. InTech, 2015, ISBN: 978-953-51-2141-1, DOI: 10.5772/59870, 79-116.
Functionalised Nanoliposomes for Construction of Recombinant Vaccines: Lyme Disease as an Example
Turanek J., Masek J., Krupka M., Raska M. 2015. In: Matthias, G: Molecular Vaccines: From Prophylaxis to Therapy, 2019. ISBN 978-3-319-00978-0, DOI: 10.1007/978-3-319-00978-0, 561-577.
Application of Liposomes for Construction of Vaccines
Turanek J., Masek J., Raska M., Ledvina M. In: Ghista, DN: Biomedical Science, Engineering and Technology. InTech, 2011, ISBN: 978-953-307-471-9, DOI: 10.5772/1020, 653-678.