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Mašek Josef

PharmDr. Josef Mašek, Ph.D.

head of the group

Research activities

NANOPARTICLES AND NANOFIBRES AS CARRIERS OF ACTIVE SUBSTANCES

PREPARATION AND CHARACTERIZATION OF LIPOSOMES AND POLYMERAN NANOPARTICLES

LIPOSOM-BASED VACCINE FORMULATION AND TESTING

DRUG TARGETING, DIAGNOSTICS AND TERANOSTICS

DEVELOPMENT OF NEW ORAL MUCOADHESIVE DRUG FORMS

CONFOCAL MICROSCOPY

Project involvement

FIT (Pharmacology, Immunotherapy, nanoToxicology)

OP RDE project of the Ministry of Education, Youth and Sports of the Czech Republic – Reg.No. CZ.02.1.01/0.0/0.0/15_003/0000495

NV16-30299A - Nanoliposomal systems for rapid diagnosis of thrombi by MRI

AZV ČR NV16-30299A

Nanoliposomes for the development of recombinant vaccines and targeted immunotherapeutics

GAČR P304/10/1951 (2010-2013)

Multiepitope synthetic borreliosis vaccine for veterinary applications

TAČR TA01011165 (2011-2014)

Use of ultrasound in nanomedicine

KAN200520703 (2007-2011)

Bioactive biocompatible surfaces and new nanostructured composites for applications in medicine and pharmacy

KAN200100801 (2008-2012)

Center for studies of toxic properties of nanoparticles

CENATOX GBP503/12/G147 (2012 – 2018)

Education

2006–2013

Ph.D. in Biochemistry, Masaryk University Brno, Faculty of Science, Czech Republic

Dissertation thesis: ”Metallochelating nanoliposomes for construction of recombinant vaccines”

2009

Advanced Master´s degree (Doctor of Pharmacy – PharmD.) in Pharmacy, Pharmaceutical Technology and Galenic Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Pharmacy, Czech Republic

Advances Master´s thesis: Optimization of ciclopiroxolamine dose and dissolution profile

2005–2009

Bachelor´s degree (Bc.) in Cellular and Molecular Diagnostics, Masaryk University Brno, Faculty of Science, Czech Republic

Bachelor´s thesis: RNA Interference in Gene Theraphy

2001–2006

Master´s degree (Mgr.) in Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Pharmacy, Czech Republic

Awards

1st place in the “Discovery award” 2017

The award was given for an innovative achievement in the field of biomedicine for the work “Mucoadhesive nanofiber carrier of vaccines and therapeutic nanoparticles“

1st place in the poster section of the 5th Nano Today Conference 2017

„Sublingual delivery of therapeutic nanoparticles by means of multilayered nanofibre-based mucoadhesive film”, Hawaii, USA, 2017

1. place in the competition „Prix Sanofi de Pharmacie“, 2013

Special award in the 2013 Ministry of Agriculture Young Scientists Award

„Metallochelating nanoliposomes for construction of recombinant vaccines”, Praha, 2013

3rd place in the poster section of the international conference The 10th EFIS-EJI Tatra Immunology Conference 2012

„Preparation and characterisation of recombinant OSpC liposome-based experimental vaccine with entrapped lipophilic derivatives of norAbuMDP against Borrelia Burgdorferi”, Slovakia, 2012

1st place in the poster section of the international conference Nanocon 2009

“Preparation of monodisperse nickel-chelate nanoliposomes for immobilization of his-tag proteins: study of their structure using TEM, AFM and dynamic light scattering”, Rožnov pod Radhoštěm, 2009

Selected publications

Targeting Human Thrombus by Liposomes Modified with Anti-Fibrin Protein Binders

Petroková H., Mašek J., Kuchař M., Vítečková Wünschová A.,Štikarová J., Bartheldyová E., Kulich P., Hubatka F., Kotouček J., Knotigová P.T., Vohlídalová E., Héžová R., Mašková E., Macaulay S., Dyr J.E., Raška M., Mikulík R., Malý P., Turánek J., 2019. Pharmaceutics 11(12), 642; https://doi.org/10.3390/pharmaceutics11120642.

Proteins mimicking epitope of HIV-1 virus neutralizing antibody induce virus-neutralizing sera in mice

Kosztyu P., Kuchar M, Cerny J., Barkocziova L., Maly M., Petrokova H., Czernekova L., Liskova V., Raskova Kafkova L., Knotigova P., Masek J., Turanek J., Maly P., Raska M., 2019. EBioMedicine 47, 247-256. doi: 10.1016/j.ebiom.2019.07.015.

Application of Advanced Microscopic Methods to Study the Interaction of Carboxylated Fluorescent Nanodiamonds with Membrane Structures in THP-1 Cells: Activation of Inflammasome NLRP3 as the Result of Lysosome Destabilization

Turánek J., Knötigová P.T., Mašek J., Hubatka F., Kulich P., Kotouček J., Šimečková P., Čelechovská H., Koudelka Š., Procházka L., Bartheldyová E., Héžová R., Švadláková T., Vaškovicová N., Skoupý R., Krzyžánek V., Macaulay S., Fekete L., Machala M., Raška M., Kratochvílová I., 2019. Molecular Pharmaceutics, 2019, 168, 3441-3451.

N-oxy lipid-based click chemistry for orthogonal coupling of mannan onto nanoliposomes prepared by microfluidic mixing: synthesis of lipids, characterisation of mannan-coated nanoliposomes and in vitro stimulation of dendritic cells

Bartheldyová E., Knötigová P.T., Zachová K., Mašek J., Kulich P., Effenberg R., Zyka D., Hubatka F., Kotouček J., Čelechovská H., Héžová R., Tomečková A., Mašková E., Fojtikova M., Macaulay S., Bystrický P., Paulovičová L., Paulovičová E., Droz L., Ledvina M., Raška M., Turánek J., 2019. Carbohydrate Polymers 207, 521-532.

Hyaluronic Acid Surface Modified Liposomes Prepared via Orthogonal Aminoxy Coupling: Synthesis of Nontoxic Aminoxylipids Based on Symmetrically α-Branched Fatty Acids, Preparation of Liposomes by Microfluidic Mixing, and Targeting to Cancer Cells Expressing CD44

Bartheldyová E., Effenberg R., Mašek J., Procházka L., Knötigová P.T,, Kulich P., Hubatka F., Velínská K., Zelníčková J., Zouharová D., Fojtíková M., Hrebík D., Plevka P., Mikulík R., Miller A.D., Macaulay S., Zyka D., Drož L., Raška M., Ledvina M., Turánek J., 2018. Bioconjug Chem 29(7), 2343-2356.
doi:10.1021/acs.bioconjchem.8b00311.

Nonpyrogenic Molecular Adjuvants Based on norAbu-Muramyldipeptide and norAbu-Glucosaminyl Muramyldipeptide: Synthesis, Molecular Mechanisms of Action, and Biological Activities in Vitro and in Vivo

Effenberg R., Knotigova P.T., Zyka D., Celechovska H., Masek J., Bartheldyova E., Hubatka F., Koudelka S., Lukac R., Kovalova A., Saman D., Krupka M., Barkocziova L., Kosztyu P., Sebela M., Droz L., Hucko M., Kanasova M., Miller A.D., Raska M., Ledvina M., Turanek J. 2017. Journal of Medicinal Chemistry 60, 7745-7763.

Multi-layered nanofibrous mucoadhesive films for buccal and sublingual administration of drug-delivery and vaccination nanoparticles – important step towards effective mucosal vaccines

Mašek J., Lubasová D., Lukáč R., Turánek-Knotigová P., Kulich P., Plocková J.,
Mašková E., Procházka L., Koudelka Š., Sasithorn N., Gombos J., Bartheldyová E., Hubatka F., Raška M., Miller A.D., Turánek J., 2017. J Control Release 249, 183–195.

The Position of His-Tag in Recombinant OspC and Application of Various Adjuvants Affects the Intensity and Quality of Specific Antibody Response after Immunization of Experimental Mice

Krupka M., Masek J., Barkocziova L., Knotigova P.T., Kulich P., Plockova J., Lukac R., Bartheldyova E., Koudelka S., Chaloupkova R., Sebela M., Zyka D., Droz L., Effenberg R, Ledvina M, Miller AD, Turanek J, Raska M., 2016. Plos One 11, Article Number: e0148497.

Molecular Adjuvants Based on Nonpyrogenic Lipophilic Derivatives ofnorAbuMDP/GMDP Formulated in Nanoliposomes: Stimulation of Innate and Adaptive Immunity

Knotigova P.T., Zyka D., Masek J., Kovalova A., Krupka M., Bartheldyova E., Kulich P., Koudelka S., Lukac R., Kauerova Z., Vacek A., Horynova M.S., Kozubik A., Miller A.D., Fekete L., Kratochvilova I., Jezek J., Ledvina M., Raska M., Turanek J., 2015. Pharmaceutical Research 32, 1186-1199.

Enhancement of immune response towards non-lipidized Borrelia burgdorferi recombinant OspC antigen by binding onto the surface of metallochelating nanoliposomes with entrapped lipophilic derivatives of norAbuMDP

Krupka M., Masek J., Bartheldyova E., Knotigova P.T., Plockova J., Korvasova Z., Skrabalova M., Koudelka S., Kulich P., Zachova K., Czernekova L., Strouhal O., Horynova M., Sebela M., Miller A.D., Ledvina M., Raska M., Turanek J., 2012. J Control Release 160, 374-381.

Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged antigens: Preparation, structural study and immune response towards rHsp90

Masek J., Bartheldyova E., Turanek-Knotigova P., Skrabalova M., Korvasova Z., Plockova J., Koudelka S., Skodova P., Kulich P., Krupka M., Zachova K., Czernekova L., Horynova M., Kratochvilova I., Miller A.D., Zyka D., Michalek J., Vrbkova J., Sebela M., Ledvina M., Raska M., Turanek J., 2011. J Control Release 151, 193-201.

Book chapters

Nanofibers in Mucosal Drug and Vaccine Delivery

Mašek J., Mašková E., Lubasová D., Špánek R., Raška M, Turánek J. In: Morari Do Nascimento, G. (Ed.) Nanofibers – from Preparation to Applications. 2019, ISBN 978-953-51-6328-2.

Binding of Polysaccharides onto Liposomes and Characterization of Liposomal Preparation by Physical-Chemical and Advanced Microscopic Methods

Turanek J., Masek J., Raska M., Ledvina M., Paulovicova E., Hubatka F.,
Kotoucek J. In: Maiti, S.: Functional Polysaccharides for Biomedical Application. Elsevier, 2019.

Lipid-Based Nanoparticles and Microbublles

Turanek J., Miller A.D., Kauerová Z., Lukáč R., Masek J., Koudelka S., Raska M. In: Serra, PA: Advances in Bioengeneering. InTech, 2015, ISBN: 978-953-51-2141-1, DOI: 10.5772/59870, 79-116.

Functionalised Nanoliposomes for Construction of Recombinant Vaccines: Lyme Disease as an Example

Turanek J., Masek J., Krupka M., Raska M. 2015. In: Matthias, G: Molecular Vaccines: From Prophylaxis to Therapy, 2019. ISBN 978-3-319-00978-0, DOI: 10.1007/978-3-319-00978-0, 561-577.

Application of Liposomes for Construction of Vaccines

Turanek J., Masek J., Raska M., Ledvina M. In: Ghista, DN: Biomedical Science, Engineering and Technology. InTech, 2011, ISBN: 978-953-307-471-9, DOI: 10.5772/1020, 653-678.