Jan Kotouček PhD
Researcher
Research activities
PHYSICO-CHEMICAL CHARACTERIZATION OF NANOPARTICLES
ASYMMETRICAL FLOW FIELD-FLOW FRACTIONATION (AF4)
DYNAMIC/STATIC LIGHT SCATTERING AND SPECTROSCOPIC TECHNIQUES
SOLUBILIZATION OF ACTIVE SUBSTANCES INTO LIPOSOMES AND NANOEMULSION SYSTEMS
Project involvement
FIT (Pharmacology, Immunotherapy, nanoToxicology)
OP RDE project of the Ministry of Education, Youth and Sports of the Czech Republic – Reg.No. CZ.02.1.01/0.0/0.0/15_003/0000495
Education
2016-2020
Brno University of Technology, Faculty of Chemistry, Brno, Study program: Physical chemistry
Doctoral Thesis: Preparation and characterization of complex nanoparticles by filed-flow fractionation and advanced spectroscopic methods.
2021-2026
Brno University of Technology, Faculty of Chemistry, Brno, Study program: Chemistry for medical applications
Master’s thesis: Characterization of colloidal particles by excited-state proton transfer with the use of advanced fluorescence techniques.
Selected publications
Characterization and purification of pentameric chimeric protein particles using Asymmetric Flow Field-Flow Fractionation coupled with multiple detectors
Kotoucek J, Hezova R, Vrablikova A, Hubatka F, Kulich P, Macaulay S, Roessner D, Raska M, Psikal I, Turanek J. Analytical and Bioanalytical Chemistry. doi: 10.1007/s00216-021-03323-6
Targeting Human Thrombus by Liposomes Modified with Anti-Fibrin Protein Binders
Petroková H, Mašek J, Kuchař M, Vítečková Wünschová A, Štikarová J, Bartheldyová E, Kulich P, Hubatka F, Kotouček J, Knotigová PT, Vohlídalová E, Héžová R, Mašková E, Macaulay S, Dyr JE, Raška M, Mikulík R, Malý P, Turánek J. J.Pharmaceutics. 2019 Dec 2;11(12):642. doi: 10.3390/pharmaceutics11120642.
Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes
Svadlakova T, Hubatka F, Turanek Knotigova P, Kulich P, Masek J, Kotoucek J, Macak J, Motola M, Kalbac M, Kolackova M, Vankova R, Vicherkova P, Malkova A, Simeckova P, Volkov Y, Prina-Mello A, Kratochvilova I, Fiala Z, Raska M, Krejsek J, Turanek J. Nanomaterials. 2020 Feb 27;10(3):418. doi: 10.3390/nano10030418.
Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages
Šimečková P, Hubatka F, Kotouček J, Turánek Knötigová P, Mašek J, Slavík J, Kováč O, Neča J, Kulich P, Hrebík D, Stráská J, Pěnčíková K, Procházková J, Diviš P, Macaulay S, Mikulík R, Raška M, Machala M, Turánek J. J.Sci Rep. 2020 Mar 16;10(1):4780. doi: 10.1038/s41598-020-60284-z.
Preparation of nanoliposomes by microfluidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation
Kotouček J, Hubatka F, Mašek J, Kulich P, Velínská K, Bezděková J, Fojtíková M, Bartheldyová E, Tomečková A, Stráská J, Hrebík D, Macaulay S, Kratochvílová I, Raška M, Turánek J. Sci Rep. 2020 Mar 27;10(1):5595. doi: 10.1038/s41598-020-62500-2
N-Oxy lipid-based click chemistry for orthogonal coupling of mannan onto nanoliposomes prepared by microfluidic mixing: Synthesis of lipids, characterisation of mannan-coated nanoliposomes and in vitro stimulation of dendritic cells.
Bartheldyová E, Turánek Knotigová P, Zachová K, Mašek J, Kulich P, Effenberg R, Zyka D, Hubatka F, Kotouček J, Čelechovská H, Héžová R, Tomečková A, Mašková E, Fojtíková M, Macaulay S, Bystrický P, Paulovičová L, Paulovičová E, Drož L, Ledvina M, Raška M, Turánek J. Carbohydr Polym. 2019 Mar 1;207:521-532. doi: 10.1016/j.carbpol.2018.10.121. Epub 2018 Nov 29.
Short-course sublingual immunotherapy by mucoadhesive patch and tolerogenic particle enhanced allergen presentation.
Sørensen P, Turánek-Knotigová P, Mašek J, Kotouček J, Hubatka F, Mašková E, Kulich P, Lubasová D, Raška M, Leenhouts K, Turánek J.Clin Exp Allergy. 2021 Mar 7. doi: 10.1111/cea.13862.
Application of Advanced Microscopic Methods to Study the Interaction of Carboxylated Fluorescent Nanodiamonds with Membrane Structures in THP-1 Cells: Activation of Inflammasome NLRP3 as the Result of Lysosome Destabilization.